# Integrative Pharmacoinformatic and Experimental Validation of Indonesian Red Algae as Novel Functional Food for Anti-Gastric Cancer Agents
> Nurkolis F.

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_105018523347
Jurnal / Konferensi: Molecular Nutrition and Food Research
Tahun terbit: 2025
DOI: https://doi.org/10.1002/mnfr.70282
ISSN: 16134125
Kuartil SJR: Q1
Citations: 1

## Authors
- Nurkolis F.

## Abstract
Gastric cancer remains a major global health burden with limited therapeutic options due to resistance and toxicity of current treatments. Marine red algae-Gracilaria edulis, Eucheuma denticulatum, and Kappaphycus striatus-are underexplored sources of bioactive compounds that may offer multi-targeted, low-toxicity agents for gastric cancer therapy. This study integrated phytochemical profiling, structure-activity relationship (SAR) analysis, network pharmacology, molecular docking, molecular dynamics simulations, and in vitro validation. SAR and docking analyses showed strong affinities of key compounds, including camptothecin and 24(S),25-epoxycholesterol, with gastric cancer targets (PTGER3, TGFBR1, IGF1R, CDK6, BRD4). Molecular dynamics confirmed the stability of camptothecin-TGFBR1 interactions. Kappaphycus striatus extract (KSE) significantly downregulated TGF-β1 expression in MKN-45 cells in a dose-dependent manner. Cytotoxicity assays demonstrated that KSE, camptothecin, and 24(S),25-epoxycholesterol selectively inhibited AGS and MKN-45 gastric cancer cells while sparing normal epithelial cells. The findings highlight Indonesian red algae as promising, safe, and sustainable sources of multi-targeted anti-gastric cancer agents. In particular, KSE and its bioactive constituents exhibit both cytotoxic and epigenetic modulatory effects, underscoring their potential for future therapeutic development.

## Keywords
- Phytochemical
- Biology
- Cytotoxicity
- Red algae
- Cancer
- Traditional medicine
- Cancer cell
- Druggability
- Camptothecin
- Pharmacology
- Epigenetics
- Algae
- In vitro
- Biochemistry
- Fucoxanthin
- Green algae
- Hemolysis
- MTT assay
- Transcriptome
- Metastasis
- Functional food
- Computational biology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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