# Epidemiological study of malaria in north sulawesi, indonesia by fluorescence and giemsa staining
> Syafruddin

URL kanonis: https://discover.unhas.ac.id/publications/pub_scopus_0027058337
Jurnal / Konferensi: Japanese Journal of Medical Science and Biology
Tahun terbit: 1992
DOI: https://doi.org/10.7883/yoken1952.45.175
ISSN: 00215112
Citations: 7

## Authors
- Syafruddin

## Abstract
An epidemiological study of malaria infection was conducted in the Likupang District, Minahasa Regency, North Sulawesi Province, Indonesia, during August 2-15, 1991. In this study, 510 people of six villages, representing ages between 1 month to 84 years cooperated voluntarily. Blood smears stained with Giemsa and acridine orange (AO), revealed 33 and 83 malaria parasite positives respectively. This significant difference was due particularly to the fact that AO staining examined under either a daylight- or halogen-illuminated microscope equipped with interference filters was sensitive to detect low-density parasitemia in many subjects previously diagnosed negative by Giemsa staining in the field. The low malaria prevalence obtained by Giemsa staining may have been attributable to the lack of standard-quality diagnostic tools in the field or inadequate observation of the slides. In both staining methods, Plasmodium falciparum was found to be the predominant species, while the remainings were P. vivax or a mixture of both. Subjects infected with P. vivax revealed higher density of parasitemia and gametocytemia than those with P. falciparum.

## Keywords
- Giemsa stain
- Malaria
- Parasitemia
- Staining
- Gametocyte
- Epidemiology
- Acridine orange
- Plasmodium falciparum
- Plasmodium vivax
- Biology
- Medicine
- Pathology
- Immunology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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