PROFILE COMPOUNDS OF CENTELLA ASIATICA L. BASED ON GEOGRAPHIC LOCATION AND VARIATION OF SOLVENTS USING FTIR

Abstract

Centella asiatica L. is a plant that has long been used as herbal medicine. The plant's geographical location will affect the levels of bioactive compounds, which are the general criteria in selecting quality herbal medicinal raw materials to ensure the consistency of their efficacy. This study uses FTIR to compare the component profiles of Centella asiatica L. leaves based on geographic location and solvent variation. Extraction was carried out by the maceration method using three types of solvents with different polarity levels, namely ethanol (polar), ethyl acetate (semi-polar), and nhexane (nonpolar). Based on the results of the study, which obtained three different groups of spot stains from the appearance of TLC, the spectral results were relatively the same in each variation of the filtrate with different absorption intensity values, while for FTIR analysis combined with chemometrics, Bengo and Tombolopao locations had a similarity of 98.95% were classified in group I, the Cenrana location had a similarity of 93.85% classified in group II. In contrast, the Bungaya location had 84.85% similarity in group III. The ethyl acetate extract at the Bengo and Tombolopao locations had a 99.71% similarity in group I, and the Cenrana location had a 99.17% similarity in group II. In comparison, the Bungaya location had a similarity of 93.98% in group III, and the n-hexane extract in the Cenrana and Tombolopao locations had 99 similarities, 84% were classified in group I, the Bengo location had a similarity of 99.44%, and was classified into group II. The Bungaya location had a similarity of 97.18% in group III. The research concludes that there are differences in the profiles of Centella asiatica L. leaf compounds from the four sampling locations, with variations in the solvents

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