# Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera
> Kasturiasih N.P.

URL kanonis: https://discover.unhas.ac.id/publications/production-characterization-and-toxicity-test-of-l-asparaginase-from-vibrio-algi
Jurnal / Konferensi: Molekul
Tahun terbit: 2023
DOI: https://doi.org/10.20884/1.jm.2023.18.3.8153
ISSN: 19079761
Kuartil SJR: Q4
Citations: 1

## Authors
- Kasturiasih N.P.

## Abstract
L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.

## Keywords
- Vibrio alginolyticus
- Toxicity
- Brine shrimp
- Biology
- Enzyme assay
- Enzyme
- Cytotoxicity
- Acute toxicity
- Microbiology
- Food science
- Vibrio
- Chemistry
- Bacteria
- Biochemistry
- In vitro
- Fishery
- Genetics
- Organic chemistry

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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