Organic rice rhizosphere fungi produce cellulase and chitinase as a biological control agent

Abstract

Abstract Food crop security and security is a challenge in the agricultural sector to produce quality and healthy food products for consumption by the community. The use of synthetic fungicides to induce plant resistance negatively affects the abundance and diversity of biological agents.Cellulose enzymes and chitinase enzymes are capable lytic enzymes that degrade the cell walls of pathogenic fungi in plants. This study aims to isolate organic rice rhizosphere fungi and test their ability to produce cellulase and chitinase enzymes. Isolate the rice rhizosphere fungi using the spread-plate method, while testing the isolates producing cellulose enzymes using CMC mediaand testing isolates producing chitinase enzymes usingCDYA mediaadded with colloidal chitin. Penicillium sp. had the highest enzyme Hydrolysis Capacity (HC) value of 1.70 mm for cellulase and 1.19 mm for chitinase, followed by Paecilomyces sp. with an HC of 1.52 mm for cellulaseand 1.12 mm for chitinase. Aspergillus sp. the HC of the cellulase and chitinase was 1.10 mm and 1.04 mm, while Trichoderma sp. the cellulose and the chitinase HC were 1.03 mm and 1.02 mm.

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