# Molecular epidemiology of Plasmodium falciparum resistance to antimalarial drugs in Indonesia
> Syafruddin D.

URL kanonis: https://discover.unhas.ac.id/publications/molecular-epidemiology-of-plasmodium-falciparum-resistance-to-antimalarial-drugs
Jurnal / Konferensi: American Journal of Tropical Medicine and Hygiene
Tahun terbit: 2005
DOI: https://doi.org/10.4269/ajtmh.2005.72.174
ISSN: 00029637
Kuartil SJR: Q2
Citations: 30

## Authors
- Syafruddin D.

## Abstract
The extent of gene polymorphisms associated with resistance to chloroquine and sulfadoxine-pyrimethamine was examined in field isolates of Plasmodium falciparum from Indonesia. Eight malaria-endemic areas, representing a broad region of the western and eastern Indonesian Archipelago were surveyed. Blood from 20-50 patients was collected at each site, DNA was isolated, and the sequences of four different genes (dihydrofolate reductase [dhfr], dihydropteroate synthase [dhps], P. falciparum multidrug resistance 1 [pfmdr1], and P. falciparum chloroquine resistance transporter [pfcrt]) were analyzed using polymerase chain reaction and restriction fragment length polymorphisms to detect polymorphisms previously shown to be associated with resistance. This analysis identified polymorphisms in dhfr at 108-Asn/Thr, 16-Val, and 59-Arg. Polymorphisms in dhps were found less frequently, either 437-Gly alone or paired with 540-Glu. The pfcrt 76-Thr polymorphism was fixed in all parasite populations and pfmdr1 86-Tyr polymorphisms in all populations except in the most eastern regions. The pfmdr1 1042-Asp polymorphism occurred less frequently. These findings indicate that polymorphisms in genes associated with drug resistance in P. falciparum are found across a broad region of Indonesia.

## Keywords
- DHPS
- Dihydropteroate synthase
- Plasmodium falciparum
- Biology
- Chloroquine
- Malaria
- Restriction fragment length polymorphism
- Virology
- Dihydrofolate reductase
- Drug resistance
- Pyrimethamine
- Genotype
- Genetics
- Molecular epidemiology
- Gene
- Immunology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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