Molecular characterisation of mutations associated with resistance to first- and second-line drugs among Indonesian patients with tuberculosis

Abstract

conferred rifampicin-resistance on M. tuberculosis.Approximately 11.42% (4/35) of isolates with S315T mutation in katG led to rifampicin-resistance instead of isoniazid-resistance.The mutation in katG gene was found at various locations (P280P, G279R, E340Q, T271I, E340*stop codon, R373G, and S315N).Streptomycin-resistance was detected in 42% (21/50) of the strains, but only two strains had rrs gene mutations (G878A and/or S514R).Approximately 14% (7/50) of M. tuberculosis isolates were kanamycin-and capreomycin-resistant but did not harbour mutations in the rrs gene, while 80% (40/50) of the strains had mutations in the quinolone-resistance determining region (QRDR) of the gyrA gene (S95T, D94V, A90V, and S91P) including the pan-susceptible strain.Conclusions: Of the 50 strains analysed, most of the mutations in the rpoB gene associated with rifampicinresistance were also detected in the katG and gyrA genes.Molecular characterisation using DNA sequencing techniques is a highly sensitive approach for detecting mutations.

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