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Microsatellite Primer Design of Falcataria moluccana L for Improving Polymorphism on PCR analysis: Preeliminary Study
Iswanto I.
Aip Conference Proceedings
Abstract
The best approach to evaluate the superiority of selected plus trees is through progeny tests that can grow and have good genotypes, for that a wide genetic diversity is needed. Development of molecular markers benefits tree breeding program. The study aimed to design Simple Sequence Repeat (SSR) primers of F. moluccana. L and validate the designed primers. The research consisted of designing primer using Primer3plus, DNA isolation, evaluating DNA quality and quantity and amplifying DNA using the designed primers. SSR primers F. moluccana that successfully designed were F1R1 (466 bp) forward (AGT-TAATCGGATC-AGTTGGA) reverse (T-GAAAAGTAAG-TGGAA-666AA), F2R2 (506 bp) forward (CTGCA-TGGTTGTCTT-GTGTA) reverse (TAAGCCCAG-AATATAATTC-CC), F3R3 (617 bp) forward (CATGGGACTT-ACAGCCTTAG) reverse (ACC-TCTGATTGCT-GAACACT), and F4R4 (836 bp) forward (ACAAAGAT-GGAGAGTAA-AG) reverse (TAATTTGTA-TGCAGCGATT-G). The SSR designed primer that could amplify F. moluccana DNA were F1R1, F2R2, and F3R3. Moreover, Duabanga moluccana and Bambusa maculata were also successfully amplified by F1R1, F2R2, and F4R4. Overall, these evaluated primers can be used in further analysis on genetic diversity of F. moluccana. L, Duabanga moluccana and Bambusa maculata.