# Melittin-Induced Cell Death Through p53 and 8-OHdG in Breast Cell Cancer MCF-7
> Plasay M.

URL kanonis: https://discover.unhas.ac.id/publications/melittin-induced-cell-death-through-p53-and-8-ohdg-in-breast-cell-cancer-mcf-7
Jurnal / Konferensi: Biomedical and Pharmacology Journal
Tahun terbit: 2022
DOI: https://doi.org/10.13005/bpj/2433
ISSN: 09746242
Kuartil SJR: Q4
Citations: 5

## Authors
- Plasay M.

## Abstract
Melittin, one of the cytolytic peptides derived from bee venom, is a broad-spectrum efficacy candidate as an antibacterial, antifungal, and antitumor agent. This study demonstrates the cytotoxic effect of melittin isolated from Apis mellifera through the induction of p53 and 8-OHdG. The antiproliferative effect was evaluated against breast cell cancer MCF-7 via MTT assay, while the molecular mechanism of melittin on MCF-7 was assayed by p53 and 8-OHdG ELISA. With an IC50 value of 5.86 µg/mL ((very toxic)), the cytotoxic impact inhibits MCF-7 cell proliferation in a dose-dependent manner. Significant (p < 0.05) elevations in the level of p53 and 8-OHdG were evident in the IC50-treated cells compared to control. In conclusion, melittin may have considerable potential as a novel natural product-based for breast cancer.

## Keywords
- Melittin
- MCF-7
- Cytotoxic T cell
- MTT assay
- Cytolysis
- Cytotoxicity
- Cell growth
- Apoptosis
- Breast cancer
- IC50
- Cancer research
- Chemistry
- Cancer
- Cell
- Pharmacology
- Biology
- Immunology
- In vitro
- Medicine
- Biochemistry
- Internal medicine
- Human breast
- Peptide

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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