# Maturation of sheep oocytes with antioxidant -tocopherol which are activated by parthenogenesis
> Gustina S.

URL kanonis: https://discover.unhas.ac.id/publications/maturation-of-sheep-oocytes-with-antioxidant-tocopherol-which-are-activated-by-p
Jurnal / Konferensi: Iop Conference Series Earth and Environmental Science
Tahun terbit: 2020
DOI: https://doi.org/10.1088/1755-1315/492/1/012070
ISSN: 17551307
Citations: 3

## Authors
- Gustina S.

## Abstract
Abstract This study was conducted to investigate the competence of sheep oocytes which are matured with antioxidant α-tocopherol by parthenogenesis activation. Oocytes from slaughterhouse were matured in tissue culture medium (TCM)-199 supplemented with 10% fetal bovine serum (FBS), 10 IU/ml pregnant mare serum gonadotrophin (PMSG), 10 IU/ml human chorionic gonadotrophin (hCG), 1 µg/ml estradiol, 50 µg/ml gentamycin, and with or without 150 µM α-tocopherol supplementation, then were parthenogenetically activated by exposing to 5 µM calcium ionophore for 5 min, and in medium containing 5 µg/ml cytochalasin B for 4 h. Furthermore oocytes were cultured in TCM 199 + 10% FBS either with or without supplementation of 150 µM α-tocopherol. Result of this experiment revealed that the percentages of activated oocytes with supplementation of 150 µM α-tocopherol only in maturation medium (63.41%) significantly higher (P<0.01) than the control group (50.00%) and the supplemented only in culture medium (51.02%). Furthermore, no significantly difference (P>0.05) in the number of oocytes with 1 pronucleus (PN), 2 PN, and more than 2 PN. It is concluded that the supplementation of 150 µM α-tocopherol on the maturation medium could also increase the percentage of activated oocytes optimally.

## Keywords
- Pronucleus
- Andrology
- Tocopherol
- Vitamin E
- Parthenogenesis
- Chemistry
- Antioxidant
- Oocyte
- Endocrinology
- Biology
- Internal medicine
- Biochemistry
- Medicine
- Embryo
- Embryogenesis
- Genetics
- Zygote
- Gene

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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