Liquid chromatography-mass spectrometry characterization of bioactive compounds from sargassum polycystum with the larval and in vitro anticancer activity

Abstract

Sargassum polycystum is a type of seaweed that has been widely used in traditional medicine. This study examines the cytotoxic effect of S. polycystum extracts against A. salina and the active extract was then fractionated Liquid Vacuum Chromatography (LVC). The fraction was the evaluation of anticancer potential against lung and breast cancer cells in vitro and their chemical components of fractions by LC-MS Bruker micrOTOF-mass spectrometer (ESI -) technique. Ethyl acetate extract was very toxic activity against A. salina with LC50 values of 12.37 g/mL compared to n-hexane with LC50 values of 336.58 g/mL and methanol with LC50 values of 315.35 g/mL. The results show that ethyl acetate was obtained from a combined fraction of 4 fractions, these fractions can inhibit lung (H-460 cells) and breast cancer (MCF-7 cells) with concentrations less than 100 g/mL. Some major components are identified using LC-MS also some components have not yet been identified.

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