Lidocaine suppressed hyperinflammation in BALB/c mice model sterile injury via downregulation of toll-like receptor 4

Abstract

Background: To study the efficacy of systemic lidocaine in suppressing toll-like receptor 4 (TLR4) protein level in BALB/c mouse with sterile injury. Material and methods: Twenty healthy adult male BALB/c mice were divided into lidocaine and control groups. The sterile injury was performed by breaking the left thigh bone of the mouse without laceration. Four hours after sterile injury the lidocaine group was treated with 2 mg/kg of lidocaine through tail vein injection. The same volume of distilled water was injected into control group instead of lidocaine. Blood was drawn from tail vein before injury, 4 h after sterile injury and 2 h after systemic lidocaine and distilled water administration. TLR4 protein level was examined by enzyme-linked immunosorbent assay (ELISA). Results: The TLR4 protein level in mice that sustained hyper inflammation due to sterile injury was significantly decreased in the lidocaine group. (p < 0.00). Conclusion: Systemic therapy of lidocaine effectively inhibits TLR4 protein in BALB/c mice that sustained hyperinflammation due to sterile injury. Keywords: Lidocaine, TLR4, Sterile injury

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