# Isolation of papain-soluble collagen from the skin of snake-head fish (Channa striata)
> Ramli A.R.

URL kanonis: https://discover.unhas.ac.id/publications/isolation-of-papain-soluble-collagen-from-the-skin-of-snake-head-fish-channa-str
Jurnal / Konferensi: Canrea Journal Food Technology Nutritions and Culinary Journal
Tahun terbit: 2020
DOI: https://doi.org/10.20956/canrea.v3i2.367
ISSN: 26219468
Kuartil SJR: Q3
Citations: 9

## Authors
- Ramli A.R.

## Abstract
Snake-head fish (Channa striata) skin categorized a byproduct contains a higher concentration of collagen. The collagen can be extracted by using protease, which is known as Papain Soluble Collagen (PaSC). This study aimed to isolate the collagen from the snake-head fish skin using papain. The yield percentage and moisture content of PaSC in the skin was determined. Measurements using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) were performed to characterize the PaSC and its functional groups. The results showed that the yields and moisture content of PaSC were 8.9% and 6.07%, respectively. The PaSC characterized by two α-chains appeared as collagen type I. Fourier transform infrared (FTIR) spectra of PaSC confirmed a triple-helical structure of collagen. The results indicated that snake-head fish skin could be used as potential resources of collagen and papain can be used as an alternative affordable enzyme.

## Keywords
- Papain
- Fourier transform infrared spectroscopy
- Chemistry
- Fish <Actinopterygii>
- Polyacrylamide gel electrophoresis
- Protease
- Chromatography
- Biochemistry
- Biology
- Enzyme
- Fishery
- Physics
- Quantum mechanics

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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