Isolation of fungi producing hormone Indole Acetic Acid (IAA) on sugarcane bagasse and filter cake

Abstract

Abstract Sugarcane bagasse and filter cake are the residual results of the process of extracting sugarcane stems into sugar which is widely used as an organic material that is beneficial to plants. The purpose of this study was to determine the ability of fungi that live in sugarcane bagasse and filter cake to produce Indole Acetic Acid (IAA) hormone. A bagasse sample of 1 gram was crushed and suspended in 99 ml of pure aquades water and a 10 gram sample of filter cake was suspended in 90 ml of pure aquades. 1 ml was taken and put into 9 ml of sterile distilled water and then mixed using vortex and diluted 10 −5 to 10 −6 , 0.1 ml pipette then poured into a cup containing Potato Dextrose Agar (PDA) media. Purification was carried out by removing a fungi colony on a sterile PDA media and then morphologically characterized. Qualitatively testing IAA production capability by culturing 1 full loop on the PDA media, then put it into PDB media and added L-Triptophan and Salkowski reagents. Supernatant was stored for 24 hours at room temperature and dark. The change in color of the superoxide to pink indicates IAA and the quantity test was performed using a spectrophotometer λ 530 nm to measure absorbance. The results show that bagasse and filter cake isolates had morphological differences. All isolates tested had the ability to produce IAA. The average production of the hormone IAA of isolate from sugarcane bagasse was 1.063 – 3.469 ppm while filter cake was 0.891-1.547 ppm. The highest IAA production produced by T4 isolates of 3.469 ppm and fungi isolates which produced the lowest IAA hormone was B21 isolates of 0.047 ppm. T4 isolates from sugarcane bagasse have good potential to be developed for use in plants.

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