Isolation and Identification of Anticancer-Protein-Producing Symbiotic Bacteria from Green Algae Caulerpa lentillifera

Abstract

Green algae symbiotic bacteria are a potential source of anticancer compounds. This study aims to identify symbiotic bacteria from Caulerpa lentillifera that have the potential to produce bioactive proteins as anticancer. Bacteria were isolated using Nutrient Agar (NA) media with pour-plate technique and purified by streak plate method. Bacteria with different morphological characteristics were subjected to biochemical tests and Gram tests. Proteins from bacterial cells were extracted with buffer A through a freeze/thaw and sonication process. Then an antioxidant test was performed using the DPPH method and a toxicity test using the Brine Shrimp Lethality Test (BSLT) method. Two isolated symbiotic bacteria, Enterobacter agglomerans (Gram-positive) and Bacillus circulans (Gram-negative) are potential sources of bioactive compounds. E. agglomerans has an IC50 of 5.04 ppm and LC50 of 11.70 ppm, whereas B. circulans has an IC50 of 6.17 ppm and an LC50 of 9.76 ppm. The two symbiotic bacteria from C. lentillifera were shown to contain proteins that have potential as anticancer agents.

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