# Isolation and Characterization of Gonad Inhibiting Hormone (GIH)-coding Gene in black tiger shrimp (Penaeusmonodon Fabricius, 1798)
> Trijuno D.D.

URL kanonis: https://discover.unhas.ac.id/publications/isolation-and-characterization-of-gonad-inhibiting-hormone-gih-coding-gene-in-bl
Jurnal / Konferensi: Iop Conference Series Earth and Environmental Science
Tahun terbit: 2021
DOI: https://doi.org/10.1088/1755-1315/763/1/012036
ISSN: 17551307
Citations: 1

## Authors
- Trijuno D.D.

## Abstract
Abstract One of the methods to increase the production in crustaceans is a highly complex hormonal process that requires precise coordination of external and internal factors to be successful. One of the important peptide hormones controlling reproduction in crustaceans is the gonad-inhibiting hormone (GIH). This gene is believed to be capable of encoding important components of the reproduction process, primarily inhibiting gonadal maturation in shrimp. As a key component in understanding the reproduction of Penaeus monodon , we characterised the expression pattern of the GIH gene in the eyestalks. This study aimed to isolate and characterize the gonad inhibiting hormone-encoding gene. The GIH gene was isolated using PCR with GIH-F and GIH-R primers. The PCR products (cDNA fragments) were sequenced. Sequence analysis was conducted in GENETYX version 7 and BLAST-N (basic local alignment search tool-nucleotide) was used to determine the similarity of sequences generated with existing sequences in GenBank. The cDNA sequences were 316 bp, and had 96%-99% identity with P. monodon GIH accessions in GenBank. We conclude that the PCR amplified cDNA fragments of the GIH encoding gene sequences from the shrimp.

## Keywords
- Penaeus monodon
- Biology
- Complementary DNA
- Gonad
- Gene
- GenBank
- Shrimp
- Genetics
- Coding region
- Gene family
- Molecular biology
- Gene expression
- Endocrinology
- Fishery

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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