Isolation and Characterization of Gonad Inhibiting Hormone (GIH)-coding Gene in black tiger shrimp (Penaeusmonodon Fabricius, 1798)

Abstract

Abstract One of the methods to increase the production in crustaceans is a highly complex hormonal process that requires precise coordination of external and internal factors to be successful. One of the important peptide hormones controlling reproduction in crustaceans is the gonad-inhibiting hormone (GIH). This gene is believed to be capable of encoding important components of the reproduction process, primarily inhibiting gonadal maturation in shrimp. As a key component in understanding the reproduction of Penaeus monodon , we characterised the expression pattern of the GIH gene in the eyestalks. This study aimed to isolate and characterize the gonad inhibiting hormone-encoding gene. The GIH gene was isolated using PCR with GIH-F and GIH-R primers. The PCR products (cDNA fragments) were sequenced. Sequence analysis was conducted in GENETYX version 7 and BLAST-N (basic local alignment search tool-nucleotide) was used to determine the similarity of sequences generated with existing sequences in GenBank. The cDNA sequences were 316 bp, and had 96%-99% identity with P. monodon GIH accessions in GenBank. We conclude that the PCR amplified cDNA fragments of the GIH encoding gene sequences from the shrimp.

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