Identification of ß-Catenin Gene as a Colorectal Cancer Controller in Mice (Mus musculus) Induced by Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS) Using PCR RFLP Method with EcoR1 and Hinif1

Abstract

Colorectal cancer is cancer attacking the colon to the rectum. The pathophysiology of colorectal cancer occurs due to several causes, such as changes in normal colonic epithelial cells histopathologically through molecular processes. Another cause is that the adenomatous polyps become colorectal cancer due to the carcinogenesis process. Most colorectal cancers originate from adenocarcinomas. Colon cancer is characterized by the uncontrolled growth of cells in the epithelial lining of the large intestine. The type of this study was laboratory experimental research. The population was 2 mice that had been induced by Azoxymethane (AOM) and Dextran sulfate sodium (DSS) and 1 mouse did not get any treatment for 2 months. From the result, the results of RFLP on PCR products from 3 samples that had been used showed that only one sample showing the presence of the β-catenin gene by marking the formation of a 227bp Deoxy Nucleic Acid (DNA) band and testing the EcoR1 restriction enzyme did not show any cutting of DNA fragments with no DNA bands. The size of 81bp and 146bp for Hinf1 restriction showed a mutation in P3 with the formation of bands of 89bp and 138bp in the large intestine that had been induced by azoxymethane and dextran sodium sulfate. Through this study, it can be seen that the occurrence of mutations in the-catenin gene as a marker of colorectal cancer can be identified using the Hinif1 enzyme with the RFLP PCR method.

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