First DNA barcodes of two endemic fishes from the Maros karst region, South Sulawesi, Indonesia

Abstract

Sulawesi is the largest island in the Wallacea biodiversity hotspot. However, data on the ichthyofauna of this island are incomplete, and several known species do not have reference DNA barcodes in public databases; these are necessary, inter alia, to enable non-invasive environmental DNA detection. As a contribution towards filling this gap, specimens of two species endemic to South Sulawesi were collected from the Maros karst region in March 2023: Dermogenys orientalis and Lagusia micracanthus. Right pectoral fins were preserved in 96% ethanol for DNA barcoding before preservation of three voucher specimens from each species at Hasanuddin University. DNA extraction and PCR (COI mitochondrial genetic marker) was followed by Sanger sequencing. Data analysis was conducted in MEGA 11. These first barcodes were deposited in GenBank: Dermogenys orientalis accessions OR829330- OR829332 and Lagusia micracanthus accessions OR829336-OR829336. Phylogenetic trees were constructed using homologous sequences obtained through the NCBI GenBank BLASTn routine.

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