# Epidemiology of Plasmodium infections in Flores Island, Indonesia using real-time PCR
> Kaisar M.M.

URL kanonis: https://discover.unhas.ac.id/publications/epidemiology-of-plasmodium-infections-in-flores-island-indonesia-using-real-time
Jurnal / Konferensi: Malaria Journal
Tahun terbit: 2013
DOI: https://doi.org/10.1186/1475-2875-12-169
ISSN: 14752875
Kuartil SJR: Q1
Citations: 21

## Authors
- Kaisar M.M.

## Abstract
BACKGROUND: DNA-based diagnostic methods have been shown to be highly sensitive and specific for the detection of malaria. An 18S-rRNA-based, real-time polymerase chain reaction (PCR) was used to determine the prevalence and intensity of Plasmodium infections on Flores Island, Indonesia. METHODS: Microscopy and real-time multiplex PCR for the detection of Plasmodium species was performed on blood samples collected in a population-based study in Nangapanda Flores Island, Indonesia. RESULTS: A total 1,509 blood samples were analysed. Real-time PCR revealed prevalence for Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae to be 14.5%, 13.2%, and 1.9% respectively. Sub-microscopic parasitaemia were found in more than 80% of all positive cases. The prevalence of P. falciparum and P. vivax was significantly higher in subjects younger than 20 years (p ≤ 0.01). In the present study, among non-symptomatic healthy individuals, anaemia was strongly correlated with the prevalence and load of P. falciparum infections (p ≤ 0.01; p = 0.02) and with the load of P. vivax infections (p = 0.01) as detected with real-time PCR. Subjects with AB blood group tend to have a higher risk of being infected with P. falciparum and P. vivax when compared to other blood groups. CONCLUSION: The present study has shown that real-time PCR provides more insight in the epidemiology of Plasmodium infections and can be used as a monitoring tool in the battle against malaria. The unsurpassed sensitivity of real-time PCR reveals that sub microscopic infections are common in this area, which are likely to play an important role in transmission and control. TRIAL REGISTRATION: Trials number ISRCTN83830814.

## Keywords
- Parasitology
- Epidemiology
- Tropical medicine
- Public health
- Virology
- Malaria
- Plasmodium (life cycle)
- Medicine
- Plasmodium falciparum
- Biology
- Veterinary medicine
- Environmental health
- Parasite hosting
- Immunology
- Pathology
- World Wide Web
- Computer science

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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