Enzyme Activity Assay of Lactic Acid Bacteria from Civet (Paradoxurus hermaphroditus) Digestive Tract

Abstract

L actic acid bacteria (LAB) are Gram-positive bacteria without cytochromes that have a rod or round shape, non-sporing, facultatively anaerobic, cannot reduce nitrates, negative-oxidation, negative-catalase, where lactic acid fermentation is the primary metabolism (Temmerman et al., 2004; Bintsis, 2018).Lactic acid bacteria are commonly used in the food and non-food industries due to their ability to generate a variety of enzymes.In the food industry, lactic acid bacteria are widely used to produce various fermentation products (Rhee et al., 2011).In addition, lactic acid bacteria also play a role in increasing the aroma and taste of food products, for example in coffee (Pereira et al., 2020) and yogurt making (Chen et al., 2017).Lactic acid bacteria groups are found in many fermented products (Vasiljevic and Shah, 2008).In addition, the lac-research Article Abstract | Food and non-food fermentation actually use lactic acid bacteria alteration as agent in the production process for enhance the aroma and taste of the product, in the case of civet coffee.This study aimed to determine and identified the ability of enzymes produced by lactic acid bacteria from civet digestion tract.The enzymes analyzed in this study were protease, lipase, and cellulase enzymes.The activity of protease carried out by the Lowry method, the lipase activity used the titrimetric method, while the cellulase activity used dinitrosalicylic (DNS) colorimetric method.The result showed that lactic acid bacteria from civet can produced protease, lipase, and cellulase on different capacity.For the protease enzyme, the highest activity enzyme was produced by Leuconostoc pseudomesenteroides Ni1324 (0.051 U/mL), while the lowest was produced by Weissella cibaria MG5327 (0.041 U/mL).For the lipase enzyme, the highest activity enzyme was produced by Leuconostoc pseudomesenteroides Ni1324 (0.639 U/mL), while the lowest was produced by Weissella cibaria MG5327 (0.306 U/mL).And for the cellulase, the highest activity enzyme was produced by Weissella cibaria MG5327 (0.039 U/mL), while the lowest was produced by Leuconostoc pseudomesenteroides CF102 (0.029 U/mL)

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