Endophytic trichoderma species of palu valley shallot origin with potential for controlling purple blotch pathogen alternaria porri

Abstract

Endophytic strains of Trichoderma can be used as an alternative to chemicals to control plant disease.Here, the work report on isolation and identification of Trichoderma from shallot (Allium cepa var agregatum) in Palu Valley, the endophytic capability of these fungi to colonize plant tissues, then in vitro and in vivo applications of Trichoderma to control the pathogen of shallot purple blotch disease, Alternaria porri.Of three isolated, two strains (T1FLS and T3RZR) were characterized as T. asperellum and one isolate (T2RZS) as T. harzianum.All were recovered from root, stem and leaf tissues when the fungi were reintroduced into young shallot by root inoculation.In vitro application indicated that these three strains inhibited A. porri colony by 74.6, 71.4 and 70.9%, respectively, nine days of post-dual culture on PDA medium.In vivo trial showed that pretreatment of shallot with Trichoderma through roots could inhibit purple blotch intensity by 39.3, 49.1 and 3.6%, respectively, 35 days after A. porri inoculation.Therefore, the study results demonstrate the presence of endophytic Trichoderma in Palu Valley shallot crops, and especially T. asperellum strain T3RZR could potentially be used as bio-fungicide to control the purple blotch disease in the field.

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