# Ellagitannins from Punica granatum leaves suppress microsomal prostaglandin E synthase-1 expression and induce lung cancer cells to undergo apoptosis
> Toda K.

URL kanonis: https://discover.unhas.ac.id/publications/ellagitannins-from-punica-granatum-leaves-suppress-microsomal-prostaglandin-e-sy
Jurnal / Konferensi: Bioscience Biotechnology and Biochemistry
Tahun terbit: 2020
DOI: https://doi.org/10.1080/09168451.2019.1706442
ISSN: 09168451
Kuartil SJR: Q3
Citations: 29

## Authors
- Toda K.

## Abstract
Prostaglandin E<sub>2</sub> (PGE<sub>2</sub>), which is a potent pro-inflammatory lipid mediator, is biosynthesized from arachidonic acid by cyclooxygenase-2 (COX-2) and microsomal PGE synthase-1 (mPGES-1). Non-steroidal anti-inflammatory drugs (NSAIDs) are used clinically as COX inhibitors, but they have gastrointestinal and cardiovascular side-effects. Thus, the terminal enzyme mPGES-1 holds promise as the next therapeutic target. In this study, we found that the ellagitannins granatin A and granatin B isolated from pomegranate leaves, and geraniin, which is their structural analog, selectively suppressed mPGES-1 expression without affecting COX-2 in non-small cell lung carcinoma A549 cells. The ellagitannins also down-regulated tumor necrosis factor α, inducible nitric oxide synthase, and anti-apoptotic factor B-cell chronic lymphocytic leukemia/lymphoma 2, and induced A549 cells to undergo apoptosis. These findings indicate that the ellagitannins have anti-inflammatory and anti-carcinogenic effects, due to their specific suppression of mPGES-1.<b>Abbreviations</b>: Bcl-2: B-cell chronic lymphocytic leukemia/lymphoma 2; COX: cyclooxygenase; CRE: cAMP response element; DHHDP: dehydrohexahydroxydiphenoyl; Et<sub>2</sub>O: diethyl ether; EtOAc: ethyl acetate; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; iNOS: inducible nitric oxide synthase; mPGES-1: microsomal prostaglandin E synthase-1; <i>n</i>-BuOH: water-saturated <i>n</i>-butanol; NSAIDs: non-steroidal anti-inflammatory drugs; NF-κB: nuclear factor-κB; PG: prostaglandin; TNF: tumor necrosis factor; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.

## Keywords
- Nitric oxide synthase
- Chemistry
- Apoptosis
- Prostaglandin
- Prostaglandin E
- Tumor necrosis factor alpha
- Prostaglandin E2
- Terminal deoxynucleotidyl transferase
- TUNEL assay
- Biochemistry
- Pharmacology
- Biology
- Endocrinology
- Enzyme

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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