EFFICACY OF PAPAYA LEAF BIOLARVICIDE AGAINST AEDES AEGYPTI LARVAE

Abstract

Aedes aegypti is the main vector of dengue virus. The use of chemical larvicides can cause resistance and environmental pollution, so natural alternatives are needed such as papaya leaves (Carica papaya L.) which contain bioactive compounds with larvicidal potential. Methods: A quasi-experimental study with post-test only control group design was conducted at Pabaeng-Baeng Market and Hasanuddin University Phytochemical Laboratory. A total of 450 instar III-IV larvae were exposed to papaya leaf extract concentrations of 10, 20, 30%, negative control (distilled water), positive control (temephos), and 96% ethanol. Mortality was observed up to 24 hr, analyzed by ANOVA and probit analysis. The LC50, LC90, LC99 values were 29.995 ppm; 58.241 ppm; and 100.040 ppm, respectively, with LT50, LT90, LT99 of 32.314; 70.687; and 133.811 min. Mortality increased with concentration with significant differences between treatments (p< 0.05). Papaya leaf extract is effective as an environmentally friendly biolarvicide against Aedes aegypti larvae, although it requires higher concentrations in the field.

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