Effects of Sorbitol and Butylated Hydroxytoluene on Quality, Lipid Peroxidation and Intracellular Calcium Concentration of Gaga Chicken Frozen Sperm

Abstract

Gaga chicken is a native Indonesian breed, requiring preservation through sperm cryopreservation. In this process, the addition of sugar in sperm diluent serves as a cryoprotectant, and the incorporation of antioxidants prevents lipid peroxidation in sperm. Therefore, this study aimed to investigate the effect of adding sorbitol, butylated hydroxytoluene (BHT), and the combination in sperm diluent on sperm quality, lipid peroxidation, and intracellular calcium (Ca2+) concentration in Gaga chicken frozen sperm. Pooled sperm was collected from male Gaga chickens and divided into 4 tubes. Each tube represented a different treatment, containing Ringer’s lactate egg yolk (RLEY) diluent alone, RLEY with 3 mM BHT, RLEY with 2% sorbitol, and RLEY with 3 mM BHT and 2% sorbitol. Sperm was put in mini straws, equilibrated, pre-freezed, and stored frozen. Motility, kinematics, viability, sperm Ca2+ concentration, and malondialdehyde (MDA) concentration were measured post-thawing. The results showed that treatment had a significant effect (P < 0.05) on sperm motility, various kinematic parameters, viability, Ca2+ concentration, and post-thawing MDA concentration. In post-thawing, progressive motility and viability were highest in P2 and P3 diluents, while the lowest was observed in Ca2+ and MDA concentrations. The addition of sorbitol in the diluent produced the bestsperm quality, maintaining intracellular Ca2+ concentration and preventing lipid peroxidation in Gaga chicken frozen sperm.

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