Effects of increasing temperature and nitrate concentration on cell abundance, growth rate, biomass and free fatty acid of Tetraselmis sp

Abstract

Abstract It is well known that marine microalgae have a high diversity and potency as natural food for aquaculture, a high bioactive compound, and source of biofuel. Tetraselmis sp. is one of the marine-green algae, which has a high lipid content. A completely randomized design was used in this experiment with four levels of temperature treatments (28°C, 30°C, 32°C, and 34°C) and three levels of nitrate treatments (0 gr/L, 0.2 gr/L and 0.4 gr/L) with length of culture was 21 days. The result of our study showed that the highest growth rate was found at 30°C with double nitrate concentration account for 0.20 cell/day. The highest biomass was found at 0.20 g/L nitrate treatment for 34°C temperature treatment account for 0.17 g/L, while the lowest dry weight was 0.08 g/L that was found at 0.0 g/L nitrate treatment for 28°C temperature treatment. The highest free fatty acid percentage was found at 30°C of temperature treatment at the 0.40 g/L of nitrate concentration treatment, account for 8.81 %. Statistically, results showed that increasing temperature affected significantly to biomass and free fatty acid while increasing nitrate was different significantly on growth rate, and biomass.

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