Effect of Adding Green Tea Polyphenols to Bali Bull Spermatozoa Diluent: Implications for Semen Quality, Hydrogen Peroxide Concentration, and Fertilization Rate

Abstract

Green Tea Polyphenols (GTP) are bioactive compounds found in green tea and are known to have strong antioxidant properties. This study aims to determine the effectiveness of adding GTP in the extender in terms of quality, hydrogen peroxide (H2O2) concentration, and fertilization rate of spermatozoa in vitro. Semen samples were collected from Bali bull using the artificial vagina method. The collected semen was diluted with treatments without the addition of GTP (T0), T1 (0.01 mg/100 mL), T2 (0.02 mg/100 mL), and T3 (0.03 mg/100 mL) GTP with 5 repetitions. The parameters tested were motility viability, Acrosome integrity, Plasma membrane integrity, DNA fragmentation, fertilization rate, spermatozoa H2O2 concentration, and oocyte H2O2 concentration. The research results show that the addition of GTP to semen diluent significantly affects viability, acrosome integrity, plasma membrane integrity, DNA fragmentation, and concentration of H2O2 (P<0.05). It does not affect motility and sperm abnormalities (P>0.05). Adding GTP also significantly affects the fertilization rate of oocytes and the concentration of H2O2 in Bali cattle oocytes (P<0.05). Adding 0.02 mg/100mL GTP to the extender yielded the best results in improving sperm quality and fertilization ability in vitro. The study's conclusion on adding GTP to the extender provides positive implications for semen quality, reduces H2O2, and improves spermatozoa fertility. Keywords: Beef cattle, artificial insemination, genetic improvement, cryopreservation, semen quality, ROS.

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