Diversity Analysis of Convergent-Derived Breeding Maize Inbred Lines using SSR Markers

Abstract

This study investigates the genetic diversity of maize (Zea mays L.) inbred lines derived from convergent breeding using Simple Sequence Repeat (SSR) markers.Convergent breeding combines desirable traits from diverse genetic sources into a single genotype, enhancing maize productivity, adaptability, and resilience.The experiment was conducted at the BSIP experimental field in Maros, South Sulawesi, and involved 30 inbred lines of maize.DNA isolation was performed using the CTAB buffer-modified extraction method, and 50 SSR markers were used to analyze genetic diversity.The results revealed significant genetic variation among the maize inbred lines.The study identified 421 alleles, with an average of 8.42 alleles per marker, indicating robust polymorphism levels.The phylogenetic analysis grouped the inbred lines into five clusters, with similarity coefficients ranging from 0.57 to 0.85, reflecting varying degrees of genetic diversity.The findings underscore the importance of using SSR markers to detect genetic diversity in maize populations, particularly those derived from convergent breeding, to enhance breeding programs and develop superior maize varieties.These findings provide valuable insights into breeding programs aiming to improve agronomic traits and to develop exceptional and adaptive maize varieties from convergent-derived inbred lines.

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