Differentiation of the DMBA Signaling Pathway on the Development of Skin Tumor Based on Histopathological Changing

Abstract

Because they develop into carcinomas and manifest in invasive and metastatic cancers, studies applying animal models have been performed continually over the years.Due to the long time needed to conduct its use and the high mortality rate of experimental animals, it frequently becomes an obstacle to efficient research.In this study, mice were administered 100 mg of DMBA (7,12-dimethylbenz [a] anthracene) twice daily to rub on the skin following it was dissolved in 0.5 ml of acetone.Methods: Two observations were carried out for the study; the first received 100 ug of DMBA twice daily for three weeks (50 ug in the morning and 50 ug in the afternoon), and the second used the same dose for 5 weeks.Results: Squamous cell carcinoma and malignant skin tumors had been identified by histological examination 3 weeks after DMBA intervention, while the same results were found 5 weeks afterwards.In this study, a malignant skin tumor in the form of squamous cell carcinoma became apparent in three and five weeks following applying the 100 ug of DMBA twice daily.Conclusion: After 3 weeks, a malignant skin tumor in the form of squamous cell carcinoma was found in an animal model applying DMBA at a dose of 100 ug twice a day.

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