Developmental Ability and Concentration of Hydrogen Peroxide in Bali Cattle Embryos Cultured In Vitro

Abstract

One of the efforts to support the productivity of Bali cattle is to utilize ovaries from the slaughterhouse by using assisted technology, namely in vitro embryo production.This study aims to determine the developmental ability and to measure the concentration of hydrogen peroxide (H 2 O 2 ) in Bali cattle embryos produced in vitro.542 oocytes were matured in TCM 199 medium, were fertilized and were cultured using CR1aa medium for 48 h.After 48 h of culture, the embryo developed into 2, 4, 8 and 16 cells.Furthermore, it was grouped based on the stage of development, each of which was re-cultured for 48 h.After that, its development was re-evaluated and H 2 O 2 measurements were taken.The 2-cell embryo was not able to reach the morula, while stage 4-, 8-and 16-cell were able to develop to the morula stage as much as 6(9.83%), 5(12.83%) and 4(25%), respectively.The concentration of H 2 O 2 in each group of cells did not differ significantly (P>0.05).However, the results of the correlation test between the H 2 O 2 concentration and the embryo's ability to reach the morula stage were negatively correlated.The lower the concentration of H 2 O 2 , the higher the chances of the embryo reaching the morula stage.The conclusion is that the high H 2 O 2 can inhibit the development of Bali cattle embryos that are cultured in vitro.

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