# Development of a reverse transcription loop-mediated isothermal amplification assay with novel quantitative pH biosensor readout method for SARS-CoV-2 detection
> Astari D.E.

URL kanonis: https://discover.unhas.ac.id/publications/development-of-a-reverse-transcription-loop-mediated-isothermal-amplification-as
Jurnal / Konferensi: APMIS
Tahun terbit: 2024
DOI: https://doi.org/10.1111/apm.13415
ISSN: 09034641
Kuartil SJR: Q1
Citations: 2

## Authors
- Astari D.E.

## Abstract
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a molecular amplification method that can detect SARS-CoV-2 in a shorter time than the current gold-standard molecular diagnostic reverse transcription-polymerase chain reaction (RT-PCR). However, previously developed RT-LAMP assays have mostly relied on highly subjective visual colorimetric interpretation. In this study, an RT-LAMP assay was developed with quantitative measurement of reaction pH using a novel portable pH biosensor compared to qualitative colorimetric interpretation and gel electrophoresis, with 57 clinical COVID-19 samples used for validation of the test. The LoD of the assay is 10<sup>3</sup> copies/μL. The highest sensitivity was found in the qualitative methods (93.75%), while the highest specificity and likelihood ratio was found in the pH sensor (87.5% and 6.72). On the sensor measurement, a significant difference (p < 0.0001) was observed between the average pH of the RT-PCR (+) COVID-19 (6.15 ± 0.27), while the average pH of the RT-PCR (-) samples (6.72 ± 0.22). Correlation analysis revealed a strong correlation (r = 0.78, p < 0.0001) between the Ct values obtained from RT-PCR with the biosensor pH readout. RT-LAMP with the quantitative pH sensor readout method has the potential to be further developed as an objective molecular assay for rapid and simple detection of SARS-CoV-2.

## Keywords
- Loop-mediated isothermal amplification
- Reverse Transcription Loop-mediated Isothermal Amplification
- Molecular biology
- Chemistry
- Real-time polymerase chain reaction
- Reverse transcription polymerase chain reaction
- Chromatography
- Reverse transcriptase
- Biosensor
- Polymerase chain reaction
- Analytical Chemistry (journal)
- Biology
- DNA
- Messenger RNA
- Biochemistry
- Gene

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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