Development and testing of PHYA gene-specific primers in sorghum to detect light-tolerant traits

Abstract

Abstract The PHYA gene is one of the genes that play a role in the shade avoidance mechanism. The study aimed to design specific primers for the PHYA gene in sorghum plants and optimize the DNA amplification process. Primers were designed using the Primer3 Plus online tool, and primers were generated and tested on 14 Indonesian sorghum varieties. Gene amplification with PCR technology requires PHYA-specific primers. The amplification rate reached 100% with aneling temperatures ranging from 54-55°C specific to each primer. The resulting band was bright, firm, and specific, and the amplicon size was on target (± 900 bp). The resulting primers can be used to amplify the PHYA gene in sorghum plants, which are then used for the sequencing process so that the base sequence of the PHYA gene in sorghum plants can be known.

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