Correlation of the Quality of Sexed-Spermatozoa of Polled Bali Bull and Protein Molecular Weight

Abstract

Polled Bali cattle are a species of Bali cattle that are naturally hornless and whose productivity can be increased by artificial insemination (AI) using sexing semen so that the sex of the calf can be controlled according to the breeder's wishes.This research used sexed spermatozoa from polled Bali cattle originating from three bulls.Motility and kinematics of spermatozoa were evaluated by computer-assisted semen analysis (CASA), viability and abnormalities were evaluated by eosin-nigrosine staining, intact plasma membrane (IPM) by HOS Test, acrosome status by FITC-PNA-PI, DNA fragmentation by AO Fluorescence, and molecular weight by SDS-PAGE.The results showed that motility, viability, and IPM between the upper and lower fractions were significantly different (P<0.05),while abnormalities, acrosome integrity, and DNA fragmentation were not significantly different (P>0.05).The results of linearity analysis showed that several protein bands in X and Y spermatozoa in the range 12-70 kDa had a positive correlation between protein molecular weight and motility, viability, abnormalities and IPM but negatively correlated with acrosome integrity and DNA fragmentation.The decrease in spermatozoa quality was thought to be caused by physical injury to spermatozoa due to the sexing process.It can be concluded that the protein bands of X and Y spermatozoa of polled Bali bulls were positively correlated with motility, viability, abnormalities and IPM, but negatively correlated with acrosome integrity and DNA fragmentation.

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