Comparison of microwave assisted extraction (MAE) with variations of power and infusion extraction method on antibacterial activity of rosella calyx extract (Hibiscus sabdariffa)

Abstract

This study aims to determine the effect of Microwave Assisted Extraction (MAE) extraction and infusion on total phenolic and antibacterial activity of rosella calyx. Extraction using Infusion and Micro Assisted Extraction methods with variations in power of 10, 30 and 50. Followed by measurement of total phenolic content and its ability to inhibit test bacteria Staphylococcus aureus and Escherichia coli. The results of the evaluation revealed that the percentage of yield of roselle calyx extract produced from the MEA method was 10, 30 and 50, respectively at 24.6%, 30.5% and 35.6% compared to the infusion method which was only 19, 5% The phenolic content of rosella calyx extract from infusion method was 2.39%, while for MAE 10, 30 and 50 respectively were 2.72%, 2.53% and 2.18%. The results of extraction using infusion had inhibition zone diameters of 11.09 ± 0.96 mm against E. coli bacteria and 6.87 ± 0.05 mm against S. aureus which had the smallest resistance of all tests. While the results of extraction with the help of microwaves obtained the highest antibacterial activity at 30 power against S. Aureus at 12.23 ± 1.85 mm compared with all extract tests from the MAE method.

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