# Comparison of Commercial Enzyme-Linked Immunosorbent Assay and Immunofluorescence Assay for Diagnosis of Acute Rickettsia typhi Infections
> Lokida D.

URL kanonis: https://discover.unhas.ac.id/publications/comparison-of-commercial-enzyme-linked-immunosorbent-assay-and-immunofluorescenc
Jurnal / Konferensi: Vector Borne and Zoonotic Diseases
Tahun terbit: 2020
DOI: https://doi.org/10.1089/vbz.2019.2451
ISSN: 15303667
Kuartil SJR: Q2
Citations: 12

## Authors
- Lokida D.

## Abstract
Murine typhus is a tropical disease caused <i>by Rickettsia typhi</i> and is endemic in resource-limited settings such as Southeast Asian countries. Early diagnosis of <i>R. typhi</i> infection facilitates appropriate management and reduces the risk of severe disease. However, molecular detection of <i>R. typhi</i> in blood is insensitive due to low rickettsemia. Furthermore, the gold standard of sero-diagnosis by immunofluorescence assay (IFA) is cumbersome, subjective, impractical, and unavailable in many endemic areas. In an attempt to identify a practical diagnostic approach that can be applied in Indonesia, we evaluated the performance of commercial <i>R. typhi</i> IgM and IgG enzyme-linked immunosorbent assay (ELISA) and IFA using paired plasma from previously studied <i>R. typhi</i> PCR-positive cases and controls with other known infections. Sensitivity and specificity of combined ELISA IgM and IgG anti-<i>R. typhi</i> using paired specimens were excellent (95.0% and 98.3%, respectively), comparable to combined IFA IgM and IgG (97.5% and 100%, respectively); sensitivity of ELISA IgM from acute specimens only was poor (45.0%), but specificity was excellent (98.3%). IFA IgM was more sensitive (77.5%), but less specific (89.7%) for single specimens.

## Keywords
- Rickettsia typhi
- Murine typhus
- Rickettsia
- Gold standard (test)
- Virology
- Immunofluorescence
- Salmonella typhi
- Biology
- Scrub typhus
- Immunology
- Antibody
- Medicine
- Microbiology
- Typhus
- Virus
- Internal medicine
- Biochemistry
- Gene
- Escherichia coli

---
Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
Saat mengutip, gunakan DOI bila tersedia atau URL kanonis di atas.