Cleavage ability of in vitro embryos of bali cattle based on different reproductive status of ovary at 48 hours after fertilization process

Abstract

Abstract This study aims to determine the ability of in vitro embryo cleavage of Bali cattle 48 hours after the fertilization process based on different ovarian reproductive status. Ovaries from abattoirs are grouped into four categories: ovaries without corpus luteum and dominant follicles (-CL and -FD), without corpus luteum and with dominant follicles (-CL and + FD), with corpus luteum and without dominant follicle (+ CL and -FD), and with corpus luteum and dominant follicles (+ CL and + FD). After that, oocytes are collected by slicing and only oocytes with a cytoplasmic are homongent and have compact cumulus cells used in the study. The selected oocytes are then matured in an incubator of 5% CO2, a temperature of 38.5 °C for 24 hours. After maturation, in vitro fertilization was carried out using fertilization media with a final concentration of spermatozoa 1.5 x 106 spermatozoa/ml. Oocytes 5-6 after fertilization were washed 3 times using CR1aa media then cultured in 5% CO2 incubator, temperature 38.5 °C for 48 hours. After 48 hours the culture was evaluated for the ability of embryo cleavage. The results showed that the number of embryos capable of cleave to stage 2 cells were not significantly different (P>0.05) in all treatments (1.64 ± 0.38% vs 8.00 ± 0.90% vs. 4.85 ± 0.96% vs. 8.22 ± 1.86%), while the number of embryos capable of cleave reached stage 4 cells (11.48 ± 0.82% vs 24.00 ± 2.64% vs. 24.27 ± 2.23% vs. 10.96 ± 0.69%), 8 cells (11.48 ± 1.15% vs 8.00 ± 1.07% vs. 26.21 ± 2.79 % vs 15.07 ± 1.40%), 16 cells (0.00 ± 0.00% vs 1.33 ± 0.38% vs. 4.85 ± 0.76% vs. 1.37 ± 0.38%), and the ability of embryos cleavage (24.59 ± 11.60% vs 41.33 ± 24.32% vs. 60.19 ± 22.45 % vs. 35.62 ± 10.83%) was significantly higher (P<0.05) in the ovaries with corpus luteum and without dominant follicles (+ CL and – FD). Conclusion, the ability of embryo division to reach 4 cells, 8 cells, and 16 cells after 48 hours culture was higher in the ovaries with corpus luteum and without dominant follicles (+ CL and – FD).

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