# Characterization and bioactivity test of secondary metabolite isolated from ethyl acetic fraction of Petrosia alfiani sponge from Barrang Lompo Island
> Rahmayanti

URL kanonis: https://discover.unhas.ac.id/publications/characterization-and-bioactivity-test-of-secondary-metabolite-isolated-from-ethy
Jurnal / Konferensi: Aip Conference Proceedings
Tahun terbit: 2020
DOI: https://doi.org/10.1063/5.0000909
Citations: 2

## Authors
- Rahmayanti

## Abstract
Sponge represent one of marine biota which very perspective as the natural compounds resource having the pharmacological activity. Characterization and bioactivity test of secondary metabolite isolated from ethyl acetic fraction of Petrosia alfiani sponge from Barrang Lompo Island has been done. The research methods included sample preparation, maceration, partition, fractionation, and purification. Isolates obtained were analyzed by TLC and its melting point was tested. Furthermore, the molecular structure was determined based on the analysis results of the spectroscopy data: IR, 13C NMR and 1H NMR. The bioactivity test was conducted towards Staphylococcus aureus, Escherichia coli, Mycobacterium tuberculosis, Candida albicans, and shrimp larvae of Artemia salina Leach. The result indicates that it is found three compounds in ethyl acetic fraction of P. alfiani sponge. Based on phytochemical test and spectroscopy was knowed that compound 1 is terpenoid, compound 2 is carboxylic acid, and compound 3 is β-sitosterol. Compound 3 can inhibit the growth of C. albicans and M. tuberculosis.

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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