# Assessment of exopolysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus ropy strain in different substrate media
> Malaka R.

URL kanonis: https://discover.unhas.ac.id/publications/assessment-of-exopolysaccharide-production-by-lactobacillus-delbrueckii-subsp-bu
Jurnal / Konferensi: Food Science and Nutrition
Tahun terbit: 2020
DOI: https://doi.org/10.1002/fsn3.1452
ISSN: 20487177
Kuartil SJR: Q1
Citations: 17

## Authors
- Malaka R.

## Abstract
The aim of this research was to determine the optimal medium for Exopolysaccharides (EPS) production by a <i>Lactobacillus delbrueckii</i> subsp. <i>bulgaricus</i> ropy strain isolated from a locally produced commercial fermented milk, in reconstituted skim milk (RSM) 10% (w/v), milk whey (MW), and soy milk whey (SMW), under optimal growth conditions for this strain. Milk whey was made by coagulating fresh milk using papaya latex 3% (v/v); soy milk whey was obtained from tofu household industry. The chemical composition of the substrate media was determined by proximate analysis, and sterilization was accomplished in an autoclave at 121°C for 15 min. Culture media were inoculated with 1% (v/v) of a starter culture of <i>L. delbrueckii</i> subsp<i>. bulgaricus</i> and then incubated at 30°C for 16 hr. EPS production, lactic acid content, cell counting, and pH were determined after the media were cooled at 5°C. Findings showed that on the basis of the growth characteristics of <i>L. delbrueckii</i> subsp<i>. bulgaricus</i>, the best medium for EPS production was RSM 10% (258.60 ± 26.86 mg/L) compared to the milk whey (69.60 ± 9.48 mg/L) and soy milk whey (49.80 ± 9.04 mg/L).

## Keywords
- Starter
- Food science
- Skimmed milk
- Lactobacillus
- Lactobacillus delbrueckii subsp. bulgaricus
- Chemistry
- Fermentation
- Lactic acid
- Lactobacillaceae
- Substrate (aquarium)
- Bacteria
- Biology
- Genetics
- Ecology

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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