# Anti-proliferating and antioxidant properties of Eriobotrya japonica fruit in human breast cancer cells combined with in silico study
> Arnamalia A.

URL kanonis: https://discover.unhas.ac.id/publications/anti-proliferating-and-antioxidant-properties-of-eriobotrya-japonica-fruit-in-hu
Jurnal / Konferensi: Cyta Journal of Food
Tahun terbit: 2024
DOI: https://doi.org/10.1080/19476337.2024.2427242
ISSN: 19476337
Kuartil SJR: Q2
Citations: 4

## Authors
- Arnamalia A.

## Abstract
This study aimed to identify compounds in the fruits of the medicinal Eriobotrya japonica L, determine anti-apoptotic properties in human breast cancer cells and predict the mechanism via docking and bioinformatic analyses. HPLC-ESI-HRMS/MS metabolite profiling of E. japonica ethanolic extract allowed the identification of eight compounds. Subsequent network pharmacology and molecular docking simulations targeting key cancer-related proteins showed excellent predictive activity (Pa > 0.71) for seven of the compounds. Two of the compounds hyperoxide (C6) and afzelin (C8) showed strong affinities with inducible nitric oxide synthase (iNOS) and beta-1 adrenergic receptor-β (ADRB1) with ΔG of −11.5 and −12.1, respectively. Radical scavenging data showed compound C8 (EC50 89.1 µg/mL, ABTS+•; 96.1 µg/mL, DPPH) was the most active in relation to other compounds and the extract (EC50 115.5 µg/mL, ABTS+•; 101.5 µg/mL, DPPH). The cytotoxicity (LD50) was 96.1–178.9 µg/mL) for cancerous MCF-7 cells compared to 1,105-2,066 µg/mL for normal MCF-10A cells.

## Keywords
- Eriobotrya
- In silico
- Japonica
- Human breast
- Antioxidant
- Cancer
- Breast cancer
- Traditional medicine
- Botany
- Biology
- Medicine
- Biochemistry
- Genetics
- Gene

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Sumber: Discover Unhas — RIMS Universitas Hasanuddin.
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