Activity and Kinetics of α-Glucosidase Inhibition by Collagen Hydrolysate from Thunnus albacares Bone

Abstract

Abstract Controlling of postprandial hyperglycemia using α-glucosidase inhibitors is one of the therapeutic approaches for the treatment of type 2 diabetes. Collagen hydrolysate, as an α-glucosidase inhibitor has not been reported. Therefore, This study intends to evaluate the activity and kinetics of inhibition of collagen hydrolysate against the α-glucosidase enzyme. Collagen hydrolysate was prepared from Thunnus albacares bone enzymatically using bacterial collagenase at hydrolysis times (0; 0.5; 1; 2; 3; 4; 5 and 6 h). The type of inhibition was determined by enzyme kinetics analysis. Collagen hydrolysate obtained at hydrolysis time of 1 h has the highest inhibitory activity of 24.47 %. Based on enzyme kinetics analysis, collagen hydrolysate showed a show a type of competitive inhibition. These results revealed that collagen hydrolysate has the potential as a natural antidiabetic in controlling blood glucose levels in type 2 diabetes.

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