Abdominal Ultrasonography Imaging after High-Fat Diet Administration in Wistar Mice (Rattus norvegicus)

Abstract

According to World Health Organization (WHO) estimates, hypercholesterolemia contributes to over four million annual fatalities and more than half of coronary heart disease cases.Alterations in dietary patterns involving highcholesterol and saturated fat foods lead to metabolic system abnormalities, impacting the digestive system, endocrine glands, reproductive system, immune system, and notably, the cardiovascular system.This study aimed to examine abdominal ultrasound imaging in Wistar mice (Rattus norvegicus) subjected to high-fat diets for 30 days to determine the concentration that can induce fat accumulation in visceral organs.The mice were categorized into five groups, each receiving a high-fat diet with varying concentrations (0.5, 1, 1.5, 2%) for 30 days.Abdominal ultrasonography was performed after the administration of the high-fat diet.The results showed that all control groups exhibited normal organ conditions.However, in the treatment groups, observations revealed no discernible boundaries of the atrial and ventricular cavities of the heart, indicating fat accumulation, visually identified as a hyperechoic appearance.In the liver, the entire treatment group displayed abnormal changes; the margins of the liver were hyperechoic in almost the entire liver, indicating fat buildup.Similarly, the spleen exhibited a hyperechoic image in almost the entire cavity.In conclusion, this study demonstrates that administering a high-fat diet comprising 0.5 to 2% of the total body weight of mice led to fat accumulation in the heart, liver, and spleen.This is characterized by a hyperechoic image, indicating fat presence and unclear boundaries between the cavities in these organs.

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